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李少伟教授博导

发布时间:2015-11-25来源:点击数:16193

 

李少伟教授

李少伟,博士  LI Shaowei, Ph.D.

教授,博士生导师 Professor

结构疫苗学 (Structural Vaccinology)

话:+86-592-2880620

真:+86-592-2181258

E-mailshaowei@xmu.edu.cn

 

简历 (Education and Work experience)

1998年,厦门大学生物化学专业,学士学位;

2003年,厦门大学生化与分子生物学/动物学专业,博士学位;

2003年,澳门新葡亰网站注册讲师;

2006年,澳门新葡亰网站注册副教授;

2011年,澳门新葡亰网站注册教授、博士生导师。

B.S.  Biochemistry, Xiamen University, 1998

Ph.D. Biochemistry&Molecular Biology/Zoology, Xiamen University, 2003

Lecturer, School of Life Sciences, Xiamen University, 2003

Associate Professor, School of Life Sciences, Xiamen University, 2006

Professor, School of Life Sciences, Xiamen University, 2011


 

研究领域 (Research Area)

? 基因工程疫苗研究与开发
? 重要传染病病毒免疫表位的结构和功能研究
? 基于结构的分子模拟和分子设计
? Research and development of recombinant human vaccine
? Structural basis and functional implication for underlying immune epitopes of key infectious viruses
? Structure-based molecular simulation and rational design for vaccine


代表性论文(Selected Publications)(* Corresponding author)

1.Zheng Q#, Jiang J#, He M#, Zheng Z#, Yu H, Li T, Xue W, Tang Z, Ying D, Li Z, Song S, Liu X, Wang K, Zhang Z, Wang D, Wang Y, Yan X, Zhao Q, Zhang J, Gu Y*, Li S*, Xia N*. Viral neutralization by antibody-imposed physical disruption. Proc Natl Acad Sci U S A. 2019 Dec 9.

2.Liu X#, Chen J#, Wang Z#, Wang D, He M, Qian C, Song S, Chi X, Kong Z, Zheng Q, Wang Y, Yu H, Zhao Q, Zhang J, Li S*, Gu Y*, Xia N. Neutralization sites of human papillomavirus-6 relate to virus attachment and entry phase in viral infection. Emerg Microbes Infect. 2019;8(1):1721-1733.

3.Chen T#, Wang K#, Chi X, Zhou L, Li J, Liu L, Zheng Q, Wang Y, Yu H, Gu Y, Zhang J, Li S*, Xia N. Construction of a bacterial surface display system based on outer membrane protein F. Microb Cell Fact. 2019 Apr 11;18(1):70.

4.Li T#, Zhang Z#, Zhang Z#, Qiao J, Rong R, Zhang Y, Yao Q, Li Z, Shen H, Huang F, Xue W, Gao S, Li S, Zheng Q, Yu H, Zhang J, Gu Y*, Li S*, Xia N. Characterization of native-like HIV-1 gp140 glycoprotein expressed in insect cells. Vaccine. 2019 Mar 7;37(11):1418-1427.

5. Wang K#, Zhou L#, Zhang X#, Song C, Chen T, Li J, Zheng M, Wang Y, Zheng Q, Zheng Z, Yu H, Wu T, Gu Y, Zhang J, Zhao Q*, Li S*, Xia N. Hepatitis E vaccine candidate harboring a non-particulate immunogen of E2 fused with CRM197 fragment A. Antiviral Res. 2019 Feb 23;164:154-161.

6.Zheng Q#, Zhu R#, Xu L#, He M#, Yan X#, Liu D, Yin Z, Wu Y, Li Y, Yang L, Hou W, Li S, Li Z, Chen Z, Li Z, Yu H, Gu Y, Zhang J, Baker TS, Zhou ZH, Graham BS*, Cheng T*, Li S*, Xia N*. Atomic structures o enterovirus D68 in complex with two monoclonal antibodies define distinct mechanisms of viral neutralization. Nat Microbiol. 2019 Jan;4(1):124-133.

7. Li Z#, Song S#, He M#, Wang D, Shi J, Liu X, Li Y, Chi X, Wei S, Yang Y, Wang Z, Li J, Qian H, Yu H, Zheng Q, Yan X, Zhao Q, Zhang J, Gu Y*, Li S*, Xia N*. Rational design of a triple-type human papillomavirus vaccine by compromising viral-type specificity. Nat Commun. 2018 Dec 18;9(1):5360.

8. Zhang Z#, He M#, Bai S#, Zhang F, Jiang J, Zheng Q, Gao S, Yan X, Li S*, Gu Y*, Xia N. T = 4 Icosahedral HIV-1 Capsid As an Immunogenic Vector for HIV-1 V3 Loop Epitope Display. Viruses. 2018 Nov 26;10(12). pii: E667.

9.Zhu R#, Xu L#, Zheng Q#, Cui Y,# Li S#, He M, Yin Z, Liu D, Li S, Li Z, Chen Z, Yu H, Que Y, Liu C, Kong Z, Zhang J, Baker TS, Yan X*, Hong Zhou Z*, Cheng T*, Xia N*. Discovery and structural characterization of a therapeutic antibody against coxsackievirus A10. Sci Adv. 2018 Sep 19;4(9):eaat7459.

10.Wei M#, Wang D#, Li Z#, Song S, Kong X, Mo X, Yang Y, He M, Li Z, Huang B, Lin Z, Pan H, Zheng Q, Yu H, Gu Y, Zhang J, Li S*, Xia N*. N-terminal truncations on L1 proteins of human papillomaviruses promote their soluble expression in Escherichia coli and self-assembly in vitro. Emerg Microbes Infect. 2018 Sep 26;7(1):160.

11. Lin Q#, Li T#, Chen Y#, Lau SY, Wei M, Zhang Y, Zhang Z, Yao Q, Li J, Li Z, Wang D, Zheng Q, Yu H, Gu Y, Zhang J, Chen H, Li S*, Xia N*. Structural Basis for the Broad, Antibody-Mediated Neutralization of H5N1 Influenza Virus. J Virol. 2018 Aug 16;92(17).

12.Zheng M#, Jiang J#, Zhang X, Wang N, Wang K, Li Q, Li T, Lin Q, Wang Y, Yu H, Gu Y, Zhang J, Li S*, Xia N. Characterization of capsid protein (p495) of hepatitis E virus expressed in Escherichia coli and assembling into particles in vitro. Vaccine. 2018 Apr 12;36(16):2104-2111.

13.Xu L#, Zheng Q#, Li S#, He M, Wu Y, Li Y, Zhu R, Yu H, Hong Q, Jiang J, Li Z, Li S, Zhao H, Yang L, Hou W, Wang W, Ye X, Zhang J, Baker TS, Cheng T*, Zhou ZH, Yan X*, Xia N*. Atomic structures of Coxsackievirus A6 and its complex with a neutralizing antibody. Nat Commun. 2017 Sep 11;8(1):505.

14.Li Z#, Wang D#, Gu Y#, Song S, He M, Shi J, Liu X, Wei S, Li J, Yu H, Zheng Q, Yan X, Baker TS, Zhang J, McLellan JS*, Li S*, Xia N*. Crystal Structures of Two Immune Complexes Identify Determinants for Viral Infectivity and Type-Specific Neutralization of Human Papillomavirus. mBio. 2017 Sep 26;8(5). pii: e00787-17.

15.Gu Y#, Wei M#, Wang D, Li Z, Xie M, Pan H, Wu T, Zhang J, Li S*, Xia N*. Characterization of an Escherichia coli-derived human papillomavirus type 16 and 18 bivalent vaccine. Vaccine. 2017;35:4637-4645.

16.Pan H#, Li Z#, Wang J, Song S, Wang D, Wei M, Gu Y, Zhang J, Li S*, Xia N*, Bacterially expressed human papillomavirus type 6 and 11 bivalent vaccine: Characterization, antigenicity and immunogenicity. Vaccine, 2017. 35(24): 3222-3231.

17. Wang D#, Fan F#, Li Z, Liu X, Song S, Wei S, He M, Lin Y, Li Z, Wei M, Yu H, Gu Y, Li S*, Xia N. Stop codon mutagenesis for homogenous expression of human papillomavirus L1 protein in Escherichia coli. Protein Expr Purif. 2017 May;133:110-120.

18. Zhang X#, Wang K#, Lin Q#, Zheng M, Li Q, Li T, Hong Q, Zheng Q, Yu H*, Gu Y, Li S*, Xia N*. A shared N-terminal hydrophobic tail for the formation of nanoparticulates. Nanomedicine, 2016 Sep;11(17):2289-303.

19.Lin Q, Yang K, He F, Jiang J, Li T, Chen Z, Li R, Chen Y, Li S, Zhao Q, Xia N. Production of Influenza Virus HA1 Harboring Native-Like Epitopes by Pichia pastoris. Appl Biochem Biotechnol. 2016 Aug;179(7):1275-89.

20. Li Z#, Yan X#, Yu H#, Wang D, Song S, Li Y, He M, Hong Q, Zheng Q, Zhao Q, Gu Y, Zhang J, Janssen M E, Cardone G, Olson N H, Baker T S*, Li S*, Xia N, The C-Terminal Arm of the Human Papillomavirus Major Capsid Protein Is Immunogenic and Involved in Virus-Host Interaction. Structure, 2016 Jun 7;24(6):874-85.

21. Gu Y, Tang X, Zhang X, Song C, Zheng M, Wang K, Zhang J,Ng MH, Hew CL, Li S*, Xia N*,Sivaraman J* .Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody. Cell Res. 2015 May;25(5): 604-20.

22. Li S, Zhang J, Xia N*. Lessons from hepatitis E vaccinedesign. Curr Opin Virol. 2015Apr; 11:130-136.

23. Wang D, Li Z, Xiao J, Wang J, Zhang L, Liu Y, Fan F, Xin L, Wei M, Kong Z, Yu H, Gu Y, Zhang J, Li S*, Xia N*. Identification of Broad-Genotype HPV L2 Neutralization Site for Pan-HPV Vaccine Development by a Cross-Neutralizing Antibody. PLoS One. 2015 Apr 23;10(4):e0123944.

24. Li S, Zhao Q, Wu T*, Chen S, Zhang J, Xia N. The development of a recombinant hepatitis E vaccine HEV 239. Hum Vaccin Immunother. 2015;11(4):908-14.

25.Gu Y, Cao F, Wang L, Hou W, Zhang J, Hew CL, Li S*, Yuan YA*, Xia N*. Structure of a Novel Shoulder-to-Shoulder p24 Dimer in Complex with the Broad-Spectrum Antibody A10F9 and Its Implication in Capsid Assembly. PLoS One. 2013 Apr 19;8(4):e61314.

26. Yang C, Pan H, Wei M, Zhang X, Wang N, Gu Y, Du H, Zhang J, Li S*, Xia N*. Hepatitis E virus capsid protein assembles in 4M urea in the presence of salts. Protein Sci. 2013 Mar;22(3):314-26.

27.Tang X, Yang C, Gu Y, Song C, Zhang X, Wang Y, Zhang J, Hew CL, Li S*, Xia N*, Sivaraman J*. Structural basis for the neutralization and genotype specificity of hepatitis E virus. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10266-71. Epub 2011 Jun 3.

28.Yu H, Li S*, Yang C, Wei M, Song C, Zheng Z, Gu Y, Du H, Zhang J, Xia N. Homology model and potential virus-capsid binding site of a putative HEV receptor Grp78. J Mol Model. 2011 May;17(5):987-95. Epub 2010 Jul 14.

29.Li S, Tang X, Seetharaman J, Yang C, Gu Y, Zhang J, Du H, Shih JW, Hew CL, Sivaraman J*, Xia N*. Dimerization of Hepatitis E Virus Capsid Protein E2s Domain is Essential for Virus-Host Interaction. PLoS Pathog. 2009 Aug;5(8):e1000537. Epub 2009 Aug 7.

30.Yang K, Li S*, He F, Zhang J, Wei M, Pan H, Yang C, Xiong J, Zhang J, Xia N. Expression and Purification of Soluble HIV-1 Envelope Glycoprotein gp160 Mutant from Saccharomyces Cerevisiae. J Biosci Bioeng. 2009 Jul;108(1):5-10.

31. Li S, Zhang J, Li Y, Ou S, Huang G, He Z, Ge S, Xian Y, Pang S, Ng M, Xia N*. A bacterially expressed particulate hepatitis E vaccine: antigenicity, immunogenicity and protectivity on primates. Vaccine. 2005 Apr 22;23(22):2893-2901.

32.Li S, Zhang J, He Z, Gu Y, Liu R, Lin J, Chen Y, Ng M, Xia N*. Mutational analysis of essential interactions involved in the assembly of hepatitis E virus capsid. J Biol Chem. 2005 Feb 4;280(5):3400-6. Epub 2004 Nov 22.



荣誉、奖励(Honors and awards)

2011年入选教育部新世纪优秀人才,2011年福建省杰出青年基金、2010年厦门市杰出青年基金;主持国家自然科学基金、“863”课题、新药创制重大专项及滚动项目等课题;获得国家科技进步二等奖、国家技术发明二等奖、中国高校科技进步一等奖、中国专利金奖和第十届紫金科技创新奖等;获授权专利13项,其中国际授权7项;入选《自然?生物技术》评出的2016年全球Top20转化研究者,是中国大陆首次入选的人员;作为主要成员研制出戊肝疫苗、宫颈癌疫苗、尖锐湿疣疫苗、艾滋诊断试剂等新药。


Top20 translational researcher, ranked by Nature Biotechnology (2016)

WIPO-SIPO Award for Chinese Outstanding Patented Invention (2012)

State Technological Invention Award (2010)

State Scientific and Technological Progress Award (2001)

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