Chuanqi Zhong, Ph.D.
2000/9-2003/6, School of Life Sciences, Wuhan University, Bachelor
2004/7-2009/9, School of Life Sciences, Wuhan University, Ph.D.
2010/3-2016/12, School of Life Sciences, Xiamen University, Postdoctor
2016/12 up to now, School of Life Sciences, Xiamen University, Associate professor
Our lab focus on the study of dynamic signaling pathway using SWATH-MS based quantitative proteomics. Signal transduction in cells was generally mediated by dynamic assembly and dissociation of protein complexes. To gain an in-depth insight into the signaling transduction in cells, we employed quantitative mass spectrometry to investigate assembly and dissociation of endogenous protein complexes.
We fist purified protein complexes using affinity purification, and subsequently identified and quantified protein components of protein complexes using SWATH-MS, which we referred to as AP-SWATH workflow. The AP-SWATH workflow can be utilized to identify novel proteins in the signaling pathway and investigate the stoichiometry of protein complexes. The AP-SWATH workflow has been successfully employed to study the TNF and LPS signaling pathways.
Selected publications (#fist author，*corresponding author）
1.Y. Li#, C.Q. Zhong#*, X. Xu, S. Cai, X. Wu, Y. Zhang, J. Chen, J. Shi, S. Lin, and J. Han* (2015). Group-DIA: analyzing multiple data-independent acquisition mass spectrometry data files. Nature methods.
2.Zhong, C. Q.#, Y. Li#, D. Yang, N. Zhang, X. Xu, Y. Wu, J. Chen and J. Han (2014). Quantitative phosphoproteomic analysis of RIP3-dependent protein phosphorylation in the course of TNF-induced necroptosis. Proteomics 14(6): 713-724.
3.Wu, X., L. Tian, J. Li, Y. Zhang, V. Han, Y. Li, X. Xu, H. Li, X. Chen, J. Chen, W. Jin, Y. Xie, J. Han* and C. Q. Zhong * (2012). Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11(12): 1640-1651.
1.Han, J., C. Q. Zhong and D. W. Zhang (2011). Programmed necrosis: backup to and competitor with apoptosis in the immune system. Nat Immunol 12(12): 1143-1149.
2.Wang, X., Gong Y, Chen Z., Gong B., Xie J., Zhong, C.Q., Wang Q., Diao L., Xu, A., Han, J., Altman A. and Li Y. (2015) “TCR-induced sumoylation of the kinase PKC-0 controls T cell synapse organization and T cell activation.” Nat. Immunol. 2015 Nov;16(11).
3.Chen, W., J. Wu, L. Li, Z. Zhang, J. Ren, Y. Liang, F. Chen, C. Yang, Z. Zhou, S. S. Su, X. Zheng, Z. Zhang, C. Q. Zhong, H. Wan, M. Xiao, X. Lin, X. H. Feng and J. Han (2015). Ppm1b negatively regulates necroptosis through dephosphorylating Rip3. Nat Cell Biol 17(4): 434-444.
4.Huang, Z., S. Q. Wu, Y. Liang, X. Zhou, W. Chen, L. Li, J. Wu, Q. Zhuang, C. Chen, J. Li, C. Q. Zhong, W. Xia, R. Zhou, C. Zheng and J. Han (2015). RIP1/RIP3 binding to HSV-1 ICP6 initiates necroptosis to restrict virus propagation in mice. Cell Host Microbe 17(2): 229-242.
5.Wu, X., W. T. He, S. Tian, D. Meng, Y. Li, W. Chen, L. Li, L. Tian, C. Q. Zhong, F. Han, J. Chen and J. Han (2014). pelo is required for high efficiency viral replication. PLoS Pathog 10(4): e1004034.
6.Tian, L., J. Chen, M. Chen, C. Gui, C. Q. Zhong, L. Hong, C. Xie, X. Wu, L. Yang, V. Ahmad and J. Han (2014). The p38 pathway regulates oxidative stress tolerance by phosphorylation of mitochondrial protein IscU. J Biol Chem 289(46): 31856-31865.
7.Chen, W., Z. Zhou, L. Li, C. Q. Zhong, X. Zheng, X. Wu, Y. Zhang, H. Ma, D. Huang, W. Li, Z. Xia and J. Han (2013). Diverse sequence determinants control human and mouse receptor interacting protein 3 (RIP3) and mixed lineage kinase domain-like (MLKL) interaction in necroptotic signaling. J Biol Chem 288(23): 16247-16261.
8.Zhong, C. Q., S. Song, N. Fang, X. Liang, H. Zhu, X. F. Tang and B. Tang (2009). Improvement of low-temperature caseinolytic activity of a thermophilic subtilase by directed evolution and site-directed mutagenesis. Biotechnol Bioeng 104(5): 862-870.
9.Fang, N., C. Q. Zhong, X. Liang, X. F. Tang and B. Tang (2010). Improvement of extracellular production of a thermophilic subtilase expressed in Escherichia coli by random mutagenesis of its N-terminal propeptide. Appl Microbiol Biotechnol 85(5): 1473-1481.
10.Yang, Y. R., H. Zhu, N. Fang, X. Liang, C. Q. Zhong, X. F. Tang, P. Shen and B. Tang (2008). Cold-adapted maturation of thermophilic WF146 protease by mimicking the propeptide binding interactions of psychrophilic subtilisin S41. FEBS Lett 582(17): 2620-2626.
11.Shi, W. L., C. Q. Zhong, B. Tang and P. Shen (2007). Purification and characterization of extracellular halophilic protease from haloarchaea Natrinema sp. R6-5. Wei Sheng Wu Xue Bao 47(1): 161-163.